A rapid, specific and economic simple UV spectrophotometric method has been developed using water and methanol (50:50, v/v) as a solvent to determine the alogliptin benzoate in bulk and pharmaceutical dosage formulation. At a predetermined λmax of 223 nm, it was proved linear in the range of 2.0 to 12.0 μg/ml and exhibited good correlation coefficient (r2 = 0.999) and excellent mean recovery (99.25-99.83%). This method was successfully applied to the determination of alogliptin benzoate content in the marketed brand and the results were in good agreement with the label claim. The method was validated statistically and by recovery studies for linearity, precision, repeatability and reproducibility. The obtained results proved that the method can be employed for the routine analysis of alogliptin benzoate in bulk as well as in the pharmaceutical dosage formulation.
Loading....